abstract：:pTK159, a multiresistance 40-kilobase (kb) plasmid, was isolated from a clinical strain of Serratia marcescens. pTK159 was nonconjugative and carried determinants for resistance to amikacin, streptomycin/spectinomycin, sulfamethoxazole and ampicillin. A physical and functional map of pTK159 was constructed. By cloning...

journal_title：Microbiology and immunology

authors： Kanno R

更新日期：1988-01-01 00:00:00

abstract：:Using anti-human B cell monoclonal antibodies prepared against B1 (CD20), B2 (CD21), B4 (CD19), and BB-1 (B lymphoblast antigen-1), we compared the expression of B cell differentiation antigens on a Jijoye-P3HR-1 cell line family of Burkitt's lymphomas. The expression of BB-1 and B2 antigens was faint on P3HR-1 K cell...

journal_title：Microbiology and immunology

authors： Yokochi T,Inoue Y,Iwata H,Miyadai T,Kimura Y

更新日期：1988-01-01 00:00:00

abstract：:An antiserum against a fraction enriched for scrapie-associated fibrils (SAF), was examined for its effects on scrapie incubation period by inoculating mice either intraperitoneally or intracerebrally with various dilutions of the serum mixed with scrapie-infected mouse brain homogenate. After intraperitoneal inoculat...

journal_title：Microbiology and immunology

authors： Ito M,Shinagawa M,Doi S,Sasaki S,Isomura H,Takahashi K,Goto H,Sato G

更新日期：1988-01-01 00:00:00

abstract：:Recombinant human interferon-gammas (rHuIFN-gamma s) were obtained from two different mammalian cells (mouse C127 cells and Chinese hamster ovary, CHO, cells) cultured in a microcarrier culture system. Both rHuIFN-gamma s were purified using sequential chromatographies for their comparison of structural properties. Th...

journal_title：Microbiology and immunology

authors： Sano E,Kamata K,Okano K,Yamazaki S,Iizuka M,Kobayashi S

更新日期：1988-01-01 00:00:00

abstract：:We investigated sex- and age-specific prevalences of hepatitis B e antigen (HBeAg) and antibody to HBeAg (anti-HBe) in 1,163 carriers of hepatitis B surface antigen (HBsAg) in Okinawa, Japan, and followed them up for longer than one year in correlation with liver function abnormalities. Elevated serum transaminase lev...

journal_title：Microbiology and immunology

authors： Hayashi J,Kashiwagi S,Ikematsu H,Nomura H,Kajiyama W,Noguchi A,Ikeda K

更新日期：1988-01-01 00:00:00

abstract：:The in vitro effect of the heat-labile toxin (HLT) of Bordetella parapertussis on HeLa, baby hamster kidney (BHK), chinese hamster ovary (CHO), myeloma (Pa-NS-1), human embryonic lung (HEL-R66) cells, erythrocytes, adipocytes and lymphocytes from guinea pigs, mice or rats, or aortic smooth muscle cells from pigs or gu...

journal_title：Microbiology and immunology

authors： Endoh M,Nagai M,Ueda T,Yoshida Y,Nakase Y

更新日期：1988-01-01 00:00:00

abstract：:The effect of human normal serum (HNS) on Pseudomonas pseudomallei was determined. It is apparent from our data that the organism is resistant to the normal serum bactericidal mechanism. Ancillary experiments to confirm this serum-resistant property of P. pseudomallei were done by examining the effects of growth phase...

journal_title：Microbiology and immunology

authors： Ismail G,Razak N,Mohamed R,Embi N,Omar O

更新日期：1988-01-01 00:00:00

abstract：:We recently developed a method for serotyping human rotavirus (HRV) by an enzyme-linked immunosorbent assay with HRV serotype-specific neutralizing monoclonal antibodies (ELISA serotyping). In the present study this method was compared with the fluorescent focus neutralization test with serotype-specific rabbit antise...

journal_title：Microbiology and immunology

authors： Urasawa S,Urasawa T,Taniguchi K,Morita Y,Sakurada N,Saeki Y,Morita O,Hasegawa S

更新日期：1988-01-01 00:00:00

abstract：:A cytotoxic T lymphocyte (CTL) response to dengue virus-infected target cells is described. Effector cells were generated in an in vitro secondary culture and appeared to be T cells possessing both the Lyt 1.1 and Lyt 2.1 surface antigens. A stronger CTL response was noted with the H-2k haplotype compared to H-2d, and...

journal_title：Microbiology and immunology

authors： Pang T,Devi S,Blanden RV,Lam SK

更新日期：1988-01-01 00:00:00

abstract：:Sera from 3,158 individuals living in northern Egypt were tested for the presence of antibodies against human T-lymphotropic virus type I (HTLV-I) by the newly developed particle agglutination (PA) test. Ten sera gave a positive reaction in the PA test. Eight of these sera were examined further by Western blotting and...

journal_title：Microbiology and immunology

authors： el-Farrash MA,Badr MF,Hawas SA,el-Nashar NM,Imai J,Komoda H,Hinuma Y

更新日期：1988-01-01 00:00:00

abstract：:The production from murine resident peritoneal macrophages (M phi) of a soluble factor, which was capable of enhancing the antigen-presenting (AP) function of dendritic cells (DC), was examined. The supernatants of peritoneal M phi (M phi sup) were prepared by culturing peritoneal M phi with particles, i.e., zymosan A...

journal_title：Microbiology and immunology

authors： Miyazaki H,Ito A,Osawa T

更新日期：1988-01-01 00:00:00

abstract：:Escherichia coli isolates from calves with diarrhea (1 day to 8 weeks old, 140 individuals) were surveyed for the three immunologically distinct fimbrial adhesins FY, 31A, and K99. Of a total of 1,370 strains isolated, 96 (7.0%), 34 (2.5%), 75 (5.5%), and 13 (0.9%) were identified as FY+, 31A+, FY+.31A+, and K99+, res...

journal_title：Microbiology and immunology

authors： Shimizu M,Sakano T,Yamamoto J,Kitajima K

更新日期：1987-01-01 00:00:00

abstract：:The biological activities of lipopolysaccharide-like substance (LLS) extracted from Leptospira interrogans serovar canicola strain Moulton by the hot phenol-water method were studied in mice. The addition of 12.5 micrograms/ml or more of LLS fraction increased the incorporation of [3H]thymidine into in vitro cultured ...

journal_title：Microbiology and immunology

authors： Shimizu T,Matsusaka E,Takayanagi K,Masuzawa T,Iwamoto Y,Morita T,Mifuchi I,Yanagihara Y

更新日期：1987-01-01 00:00:00

abstract：:Opaque (Op) and translucent (Tr) colonial variants were isolated from Vibrio vulnificus strains. Op-type variants were more resistant than the isogenic Tr-type variants, but the survival rate of the Op-type variants varied with the strains. Antisera were prepared by immunizing rabbit with whole cells of Op and Tr vari...

journal_title：Microbiology and immunology

authors： Shinoda S,Kobayashi M,Yamada H,Yoshida S,Ogawa M,Mizuguchi Y

更新日期：1987-01-01 00:00:00

abstract：:Nineteen strains of Newcastle disease virus (NDV) isolated from wild ducks in Japan were placed into 4 distinct antigenic groups on the basis of their reactivities to 8 monoclonal antibodies against the HN molecule of NDV in hemagglutination inhibition tests. The NDV strains of duck origin were antigenically distinct ...

journal_title：Microbiology and immunology

authors： Kawamura M,Nagata-Matsubara K,Nerome K,Yamane N,Kida H,Kodama H,Izawa H,Mikami T

更新日期：1987-01-01 00:00:00

abstract：:Scanning electron micrograph of HeLa S3 monolayered cells, inoculated with viable bacteria of a Salmonella typhi strain GIFU 10007, revealed that the extended microvilli tangled the bacteria within 10 min after inoculation. The micrographs of HeLa cells, at 1 hr after inoculation, indicate the following: shortening of...

journal_title：Microbiology and immunology

authors： Yokoyama H,Ikedo M,Kohbata S,Ezaki T,Yabuuchi E

更新日期：1987-01-01 00:00:00

abstract：:A human T-lymphoblastoid cell line, TCL-Fuj, produces large amounts of interferon (IFN)-gamma constitutively. A variant cell line, 2M, was derived from it. Both cell lines express similar surface antigen markers, but differ in surface morphology. Compared with the parent TCL-Fuj cell line, 2M produced less IFN-gamma c...

journal_title：Microbiology and immunology

authors： Matsuyama M,Sugamura K,Hinuma Y

更新日期：1987-01-01 00:00:00

abstract：:The distribution of the antibody to influenza C virus in the dogs and pigs in Yamagata prefecture, Japan was investigated by using three different serological methods: hemagglutination-inhibition (HI), radioimmunoprecipitation (RIP), and immunoblotting. The antibody against influenza C virus glycoprotein (gp88) was de...

journal_title：Microbiology and immunology

authors： Ohwada K,Kitame F,Sugawara K,Nishimura H,Homma M,Nakamura K

更新日期：1987-01-01 00:00:00

abstract：:The effect of the two-year milk-feeding on the gastrointestinal (GI) microflora of a cynomolgus monkey was determined. Bifidobacterium spp. in the animal fed with cow's milk alone were distributed in higher number in the stomach, duodenum, cecum, and rectum. The increasingly higher numbers of Lactobacillus spp. and Ca...

journal_title：Microbiology and immunology

authors： Benno Y,Honjo S,Mitsuoka T

更新日期：1987-01-01 00:00:00

abstract：:A protease was purified from a strain of Vibrio vulnificus isolated from the blood of a septicemic human. The vibrio was cultured in bacto peptone-yeast extract medium, and the protease was purified by a purification procedure including ultrafiltration of the culture supernatant with an Amicon YM 5 membrane, diethylam...

journal_title：Microbiology and immunology

authors： Miyoshi N,Shimizu C,Miyoshi S,Shinoda S

更新日期：1987-01-01 00:00:00

abstract：:Four N-acetylchito-oligosaccharides, from tetra-N-acetylchitotetraose (NACOS-4) to hepta-N-acetylchitoheptaose (NACOS-7), were found to increase the number of peritoneal exudate cells (PEC) in male BALB/c mice after 3 hr intraperitoneal administration of 50 mg/kg of each oligosaccharide. The number of attracted cells,...

journal_title：Microbiology and immunology

authors： Suzuki K,Tokoro A,Okawa Y,Suzuki S,Suzuki M

更新日期：1986-01-01 00:00:00

abstract：:Actin is a major antigen involved in the reaction of smooth muscle antibody positive sera from patients with chronic active hepatitis. In the present study, actin extracted from rabbit skeletal muscle was denatured by sodium dodecyl sulfate and was immunized into the rabbit, a homologous animal for actin. The rabbits,...

journal_title：Microbiology and immunology

authors： Nishioka M,Aibiki T,Shirai M,Terada S,Kagawa H,Watanabe S

更新日期：1986-01-01 00:00:00

abstract：:Twelve hybridoma cell lines producing monoclonal antibodies to the human interleukin 2 (IL-2) receptor (IL-2R) molecule were prepared. These antibodies were characterized by competitive antibody-binding assay and sequential immunoprecipitation assay with four known monoclonal antibodies to the human IL-2R molecule. Th...

journal_title：Microbiology and immunology

authors： Tanaka Y,Inoi T,Tozawa H,Sugamura K,Hinuma Y

更新日期：1986-01-01 00:00:00

abstract：:We established an assay system for detecting T cell-replacing factor (TRF) activity of translated materials in Xenopus oocytes of poly (A)-positive mRNA extracted from a T cell hybrid cell line, B151K12 (B151) which constitutively produces TRF. Since it was difficult to detect TRF activity of the translated products o...

journal_title：Microbiology and immunology

authors： Tominaga A,Matsumoto M,Takahashi T,Harada N,Takatsu K

更新日期：1986-01-01 00:00:00

abstract：:Numerical analysis of 211 strains of rapidly growing, nonphotochromogenic mycobacteria was carried out by using 116 characters. New species reported after 1981 and not yet compared with known species by numerical classification were included. At the level of 90% of the matching coefficient, the following species could...

journal_title：Microbiology and immunology

authors： Tsukamura M,Ichiyama S

更新日期：1986-01-01 00:00:00

abstract：:The heat-labile toxin (HLT) of Bordetella bronchiseptica was purified successively from sonic extracts of phase I organisms grown in Stainer-Scholte medium, by partition in hydrophobic interaction, sucrose density gradient centrifugation, gel filtration through Sepharose 4B and 6B, isoelectric precipitation and isoele...

journal_title：Microbiology and immunology

authors： Endoh M,Amitani M,Nakase Y

更新日期：1986-01-01 00:00:00

abstract：:The in vitro activity of ofloxacin, a new pyridone-carboxylic acid, against 11 strains of Chlamydia trachomatis and six strains of Chlamydia psittaci was determined. All test strains of both species were inhibited by 0.39 micrograms of ofloxacin per ml. The antichlamydial activity of ofloxacin was comparable to that o...

journal_title：Microbiology and immunology

authors： Hirai K,Une T

更新日期：1986-01-01 00:00:00

abstract：:An 8-year-old girl with meningococcal meningitis lacked serum complement activity. The seventh component of complement (C7) could not be detected in her serum by either functional or immunochemical analysis. The levels of the other components were within the normal range. Her serum complement activity was restored by ...

journal_title：Microbiology and immunology

authors： Miyake T,Ohta K,Kawamori J,Hirao T,Akagaki Y,Inai S

更新日期：1986-01-01 00:00:00

abstract：:A sensitive, quantitative, short-time, and reproducible focus assay for Japanese encephalitis (JE) virus is described. After 2 or 3 days of incubation, the infected cells were treated with anti-JE virus serum and complement, and subsequently stained with trypan blue; then clear foci were produced. This method made it ...

journal_title：Microbiology and immunology

authors： Kimura-Kuroda J,Yasui K

更新日期：1985-01-01 00:00:00

abstract：:The attachment of Mycoplasma pulmonis m53 organisms to mouse and rat synovial cells was examined by using the organisms and the synovial cells treated in various ways. M. pulmonis treated with trypsin attached to the synovial cells, but the organisms treated with pronase, formaldehyde, glutaraldehyde, or heat did not....

journal_title：Microbiology and immunology

authors： Araake M,Yayoshi M,Yoshioka M

更新日期：1985-01-01 00:00:00

abstract：:The effect of fatty acids on Mycobacterium smegmatis was examined in vitro at pH 5.0 to 7.0 to determine the role of fatty acids in the intracellular killing of mycobacteria. Unsaturated fatty acids showed strong bactericidal activity in low concentrations (0.005 to 0.02 mM), whereas saturated fatty acids, except for ...

journal_title：Microbiology and immunology

authors： Kanetsuna F

更新日期：1985-01-01 00:00:00

abstract：:Germfree (GF) F344 rats developed severe adjuvant-induced arthritis with a 100% incidence after a single intradermal injection of heat-killed Mycobacterium bovis (BCG). Specific pathogene-free (SPF) rats developed less severe arthritis with a lower incidence. The rats colonized with Escherichia coli or Bacteroides dev...

journal_title：Microbiology and immunology

authors： Kohashi O,Kohashi Y,Takahashi T,Ozawa A,Shigematsu N

更新日期：1985-01-01 00:00:00

abstract：:The mechanism of MHC-restricted T and B cell interactions in antibody response was studied with IgM-inducible B hybridomas and antigen-specific helper T cell clones. B hybridomas were prepared by fusion between splenic B cells from (CBA/N (H-2k) X BALB/c (H-2d)) F1 (NBF1) male mice and a B lymphoma cell line, M12.4.5....

journal_title：Microbiology and immunology

authors： Yoshikubo T,Kimoto M,Kishimoto T

更新日期：1985-01-01 00:00:00

abstract：:Direct stimulations of murine B lymphocytes with synthetic lipid A analogs and synthetic muramyl dipeptide (MDP) derivatives were studied using a limiting dilution assay system. Synthetic lipid A analogs, GLA-27 and GLA-40, when conjugated with bovine serum albumin (BSA) had the ability to induce B cell clonal expansi...

journal_title：Microbiology and immunology

authors： Saito-Taki T,Nakano M,Kiso M,Hasegawa A

更新日期：1985-01-01 00:00:00

abstract：:An R-form lipopolysaccharide (LPS) extracted from Klebsiella strain LEN-111 (O3-:K1-) by the phenol-chloroform-petroleum ether method was compared with that extracted by the phenol-water method in the ability to form a hexagonal assembly. The LPS which was extracted by the phenol-water method and dialyzed against tap ...

journal_title：Microbiology and immunology

authors： Kato N,Ohta M,Kido N,Ito H,Naito S,Kuno T

更新日期：1985-01-01 00:00:00

abstract：:Heat resistance at 95 C, heat activation at 75 C, and germination response were determined for spores of 10 serotype strains of Clostridium perfringens type A, including five heat-resistant and five heat-sensitive strains. The D95-values ranged from 17.6 to 63.0 and from 1.3 to 2.8 for the heat-resistant and the heat-...

journal_title：Microbiology and immunology

authors： Ando Y,Tsuzuki T,Sunagawa H,Oka S

更新日期：1985-01-01 00:00:00

abstract：:A high molecular weight proteinaceous factor in the cell extract of sarcoma 180 (S-180) was found to inhibit phorbol myristate acetate (PMA)-triggering of macrophage H2O2 release. This factor (S-180 factor) was stable at 56 C for 1 hr and resistant to ultraviolet-irradiation. The S-180 factor inhibited the specific bi...

journal_title：Microbiology and immunology

authors： Tomioka H,Saito H

更新日期：1985-01-01 00:00:00

abstract：:Molecular heterogeneity of the extracellular deoxyribonuclease (DNase) in group A streptococci was demonstrated in 42 clinical isolates. Although polyacrylamide gel electrophoretic patterns of the extracellular DNase of all the isolates were heterogeneous, they could be divided into five main patterns with respect to ...

journal_title：Microbiology and immunology

authors： Miyakawa Y,Yamada T,Shitara M,Fukazawa Y

更新日期：1985-01-01 00:00:00

abstract：:We have developed a convenient method for assaying the receptor-destroying enzyme (RDE) activity of influenza C virus. This method measures the ability of the RDE to destroy the hemagglutination-inhibition activity of a potent inhibitor present in rat serum. Some physico-chemical properties of the RDE of influenza C v...

journal_title：Microbiology and immunology

authors： Sugawara K,Kitame F,Homma M,Nakamura K

更新日期：1985-01-01 00:00:00

abstract：:Dengue virus multiplication in cultures of a murine myelomonocytic cell line (WEHI-3) as well as mouse peritoneal macrophages was enhanced by treatment of the cells with lipophilic derivatives of muramyl peptides for 2 or 3 days before virus inoculation, but not for 2 hr before virus inoculation or during the adsorpti...

journal_title：Microbiology and immunology

authors： Hotta H,Sanchez LF,Takada H,Homma M,Kotani S

更新日期：1985-01-01 00:00:00